LEVEL 06 · LESSON 03
Working volume
See it
Working volume is the volume actually placed into each culture vessel. It is distinct from the vessel's total physical capacity. A recipe scaler needs the working fill because that determines how much medium the run actually consumes.
Understand it
Record fill volume per vessel.
Use the same unit for vessel fill and total-volume calculation.
Changes in working fill can affect both total media and vessel environment.
Important boundary
Do not use nominal jar size as a substitute for actual working fill.
Try it
Explain the concept in your own words, then identify what plant, explant, stage, units, source, or observation context would need to be preserved before applying it.
Lesson quiz
Apply it
Connect this lesson to an actual MyTCLab record: what fields would you need in order to know later exactly what happened and why?