LEVEL 07 · LESSON 08

Acclimation losses

See it

Plantlets leaving in-vitro culture face major changes in humidity, light, water relations, substrate, microbes, and gas exchange. Losses during acclimatization can therefore reflect the quality of the in-vitro plantlet as well as the transition environment.

Understand it

Track survival over time, not only day-one transfer success.
Record gradual changes in humidity/light where relevant to the protocol.
Connect acclimation outcomes back to parent batch and stage history.
Important boundary

Do not evaluate acclimation without considering the condition of the plantlet before transfer.

Try it

Explain the concept in your own words, then identify what plant, explant, stage, units, source, or observation context would need to be preserved before applying it.

Lesson quiz

Apply it

Connect this lesson to an actual MyTCLab record: what fields would you need in order to know later exactly what happened and why?