LEVEL 05 · LESSON 06
Stage transitions
See it
A stage transition should be based on biological readiness and the protocol's criteria, not only on elapsed time. Useful criteria may include shoot development, rooting status, morphology, contamination status, or other plant-specific observations.
Understand it
Separate scheduled check dates from the decision to advance.
Use objective observations where possible.
Document why a culture was advanced, held, or discarded.
Important boundary
Time is a cue to inspect, not always a command to advance.
Try it
Explain the concept in your own words, then identify what plant, explant, stage, units, source, or observation context would need to be preserved before applying it.
Lesson quiz
Apply it
Connect this lesson to an actual MyTCLab record: what fields would you need in order to know later exactly what happened and why?