LEVEL 04 · LESSON 03
Vitamins and carbohydrates
See it
In-vitro cultures may receive vitamins and a carbohydrate source because their photosynthetic capacity and culture environment may not support fully autotrophic growth. Sucrose is commonly used in many protocols, but exact amounts are protocol-specific.
Understand it
Carbon source affects osmotic and metabolic conditions.
Vitamins and organic supplements vary among formulations.
Record both ingredient identity and concentration.
Important boundary
Do not generalize one carbohydrate concentration to every plant or stage.
Try it
Explain the concept in your own words, then identify what plant, explant, stage, units, source, or observation context would need to be preserved before applying it.
Lesson quiz
Apply it
Connect this lesson to an actual MyTCLab record: what fields would you need in order to know later exactly what happened and why?
