Acclimatization
By the end of this lesson
- Explain why leaving culture is a biological transition
- Identify changed environmental conditions
- Track adjustment beyond first-day survival
See it
Acclimatization transitions plantlets from the protected, humid, sugar-supported in-vitro environment toward ex-vitro conditions. In-vitro plants can have altered anatomy and physiology, making the transition a genuine biological stage rather than simple repotting.
Understand it
Explain why leaving culture is a biological transition
Humidity, light, water relations, roots, leaves, and microbial exposure all change.
Acclimatization is a biological transition involving the plantlet and its changing environment, not simply repotting.
Identify changed environmental conditions
Gradual transition is commonly important.
Several environmental and physiological factors change when a plantlet leaves the protected vessel environment.
Track adjustment beyond first-day survival
Record survival and growth after transfer, not only the day of removal from culture.
Survival immediately after removal does not demonstrate sustained adjustment or later growth.
Worked example
A plantlet survives removal from culture on day one. The grower continues observations as humidity, light and water relations change. The first successful transfer is a milestone, not proof that adjustment is complete.
Explain your decision before opening the worked reasoning below.
Show the worked reasoning
Gradual transition is commonly important because in-vitro plants may differ anatomically and physiologically from ex-vitro plants.
Tracking later performance distinguishes initial transfer success from durable acclimatization.
A plantlet that survived removal from the vessel is not automatically fully acclimatized.
Try it
What does the example's day-one survival show?
Use the worked example above to explain your answer in your own words before taking the quiz.
Lesson quiz
Assessment version: 2026-10-15-v2. Previous reading-completion markers are retained. This revised quiz has its own result.
Apply it
Use the worked example to write a short decision record: what did you observe, which rule did you apply, and what would you check before acting?
Completion marks reading progress, not a quiz pass or professional certification.